Molecular Detection of Sugarcane Ratoon Stunting Disease in Hainan Sugarcane-growing Areas

更新时间:2023-05-28

【摘要】[Objective] The paper was to clarify the occurrence of sugarcane ratoon stunting disease(RSD) in Hainan sugarcane-growing areas.[Method] In 2019, 270 samples of sugarcane leaves were collected from six main sugarcane-growing areas in Hainan Province, and RSD was detected by PCR assay with specific primers. [Result] RSD was detected out in 41 out of 270 sugarcane samples, with an average detection rate of15.19%. The detection rates of RSD were different in six sugarcane-growing areas; the detection rate of RSD in Danzhou sugarcane-growing area was the highest of 22.00%; the detection rate of RSD in Lingao sugarcane-growing area was the lowest of 9.26%. RSD was detected out in 8 out of10 main sugarcane cultivars, among which Xintaitang 22 suffered the heaviest damage, with the positive detection rate of 45.83%; RSD had not been detected out in Zhongtang 1 and Zhongtang 2, while the positive detection rates of RSD in the remaining seven sugarcane cultivars were10.00%-31.25%. [Conclusion] RSD commonly occurs in Hainan sugarcane-growing areas. The research results provide a basis for scientific prevention and control of RSD and promotion and application of healthy virus-free sugarcane seedlings.

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